PLANEACIÓN DIDÁCTICA GENERAL DE LA ASIGNATURA: CONTABILIDAD

GUÍA DIDÁCTICA / PLANEACIÓN DIDÁCTICA (NMS

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Sex in the City and Beyond: Op zoek naar sporen van prostitutie

status: publishe

Van Assepoetser tot Kung Fu Panda, Gent, 11 februari 2020

Lezing tijdens de conferentie 'Waarom leesbevordering ertoe doet', georganiseerd door de vzw Iedereen leest op 11 februari 2020.status: Published onlin

PLANEACION DE CONTABILIDAD

GUIA DIDACTICA / PLANEACION DIDACTICA (NMS